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Golden Gate Cloning
Golden Gate Cloning 〔(【引用サイトリンク】title=PLOS ONE: A One Pot, One Step, Precision Cloning Method with High Throughput Capability )〕 is a method in molecular biology that allows a researcher to simultaneously and directionally assemble multiple DNA fragments into a single piece using Type IIs restriction enzymes and T4 DNA ligase.〔(【引用サイトリンク】url=https://www.neb.com/applications/cloning-and-synthetic-biology/dna-assembly-and-cloning/golden-gate-assembly )〕 This assembly is performed ''in vitro''. Most commonly used Type IIs enzymes include BsaI, BsmBI and BbsI.
Unlike standard Type II restriction enzymes like EcoRI and BamHI, these enzymes cut DNA outside of their recognition sites and therefore can create non-palindromic overhangs. Since 256 potential overhang sequences are possible, multiple fragments of DNA can be assembled by using combinations of overhang sequences.
==References==
抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』
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